egfp dvl1  (Addgene inc)

Journal: bioRxiv

Article Title: Nup358 Sustains Intestinal Epithelial Homeostasis by Preventing Dvl1 Condensate Formation to Restrain Wnt Signaling

doi: 10.64898/2026.03.25.714063

Figure Lengend Snippet: a. Levels of the 7TGP fluorescent Wnt reporter in HEK293T cells transfected with control siRNA, Dvl1-specific siRNA, Nup358-specific siRNA, or a combination of Nup358-specific siRNA and Dvl1-specific siRNA and treated with either Wnt3a or vehicle. b. Western blot analysis and relative quantification of protein levels of Dvl1 and Axin1 in HEK293T cells transfected with either control or Nup358-specific siRNA. α-Tubulin and GAPDH were used as loading controls. Data are expressed as mean ± SD. **p ≤ 0.01. c. Immunofluorescence staining for Dvl1 and Nup358 in HEK293T cells transfected with either control or Nup358-specific siRNA. d. Immunofluorescence staining for Dvl1 and Nup358 in HEK293T cells transfected with control or Nup358-specific siRNA and treated with vehicle or 1,6-hexanediol 5% for 2 minutes. e. Fluorescence recovery after photobleaching (FRAP) and fusion analysis of Dvl1 condensates in HEK293T cells transfected with Nup358-specific siRNA and EGFP-Dvl1.

Article Snippet: Plasmids used in this study include β-catenin-EGFP (Addgene, #71367), EGFP-Dvl1 (Addgene, #194586) and plasmids HA-Nup358 1–2683 , HA-Nup358 1–2148 , HA-Nup358 1–1810 , HA-Nup358 1–1133 from Wälde et al 13 .

Techniques: Transfection, Control, Western Blot, Quantitative Proteomics, Immunofluorescence, Staining, Fluorescence

Journal: bioRxiv

Article Title: Nup358 Sustains Intestinal Epithelial Homeostasis by Preventing Dvl1 Condensate Formation to Restrain Wnt Signaling

doi: 10.64898/2026.03.25.714063

Figure Lengend Snippet: a. Schematic representing different functional domains of Nup358. b. Fluorescent Wnt reporter activity in HEK293T cells transfected with control siRNA, Nup358-specific siRNA, Ubc9-specific siRNA, or treated with the SUMOylation inhibitor 2-D08. c. Schematic representing different HA-tagged truncated forms of Nup358 that were transiently expressed in HEK293T cells. d. Fluorescent Wnt reporter activity in HEK293T cells transfected with either control or Nup358-specific siRNA alone or in combination with different HA-tagged truncated forms of Nup358 were analyzed by confocal microscopy (top) and quantified (bottom). Data are expressed as mean ± SD. ****p ≤ 0.0001 e. Western blot analysis of co-immunoprecipitation of HA-Nup358 and EGFP-Dvl1 transiently expressed in HEK293T cells. GAPDH was used as loading control. f. Immunofluorescence of HEK293T cells transfected with HA-Tagged Nup358 1–1133 and EGFP-Dvl1and stained with an anti-HA antibody. g. Immunofluorescence staining for endogenous Dvl1 and Nup358 in HEK293T cells transfected with either scramble control or Nup358-specific siRNA alone or in combination with HA-Tagged Nup358 1–1133 fragment were analyzed by confocal microscopy (top) and quantified (bottom). Data are expressed as mean ± SD. **p ≤ 0.01.

Article Snippet: Plasmids used in this study include β-catenin-EGFP (Addgene, #71367), EGFP-Dvl1 (Addgene, #194586) and plasmids HA-Nup358 1–2683 , HA-Nup358 1–2148 , HA-Nup358 1–1810 , HA-Nup358 1–1133 from Wälde et al 13 .

Techniques: Functional Assay, Activity Assay, Transfection, Control, Confocal Microscopy, Western Blot, Immunoprecipitation, Immunofluorescence, Staining

Journal: Stem Cells International

Article Title: Graphene Oxide Quantum Dots Promote Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth via the Wnt/ β -Catenin Signaling Pathway

doi: 10.1155/2021/8876745

Figure Lengend Snippet: Primer sequences used in qRT-PCR.

Article Snippet: SHEDs were seeded into a culture flask, and when they reached 50% confluence, they were treated with DMEM containing enhanced green fluorescence protein-labeled (EGFP) β -catenin small interfering RNA (siRNA) lentivirus (Shanghai Genechem Co., Ltd., Shanghai, China). siRNA against β -catenin GTATTTGAAGTATACCATA and a nonspecific shRNA construct were designed and cloned onto an hU6-MCS-CMV-EGFP vector.

Techniques: Sequencing

Journal: Stem Cells International

Article Title: Graphene Oxide Quantum Dots Promote Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth via the Wnt/ β -Catenin Signaling Pathway

doi: 10.1155/2021/8876745

Figure Lengend Snippet: (a) A green fluorescence protein marker was used to determine the transfer efficiency of β - catenin knockdown in SHEDs (SHED-si). After transfection for 72 h, cells were observed under a (A) contrast phase microscope and an (B) immunofluorescence microscope. Scale bar, 100 μ m. (b) qRT-PCR analysis was performed to detect β - catenin expression. (c) WB was conducted to determine β -catenin. (d) Quantification of β -catenin protein levels. ∗ p < 0.05 vs. the control-si group.

Article Snippet: SHEDs were seeded into a culture flask, and when they reached 50% confluence, they were treated with DMEM containing enhanced green fluorescence protein-labeled (EGFP) β -catenin small interfering RNA (siRNA) lentivirus (Shanghai Genechem Co., Ltd., Shanghai, China). siRNA against β -catenin GTATTTGAAGTATACCATA and a nonspecific shRNA construct were designed and cloned onto an hU6-MCS-CMV-EGFP vector.

Techniques: Fluorescence, Marker, Transfection, Microscopy, Immunofluorescence, Quantitative RT-PCR, Expressing

Journal: Stem Cells International

Article Title: Graphene Oxide Quantum Dots Promote Osteogenic Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth via the Wnt/ β -Catenin Signaling Pathway

doi: 10.1155/2021/8876745

Figure Lengend Snippet: SHEDs were cultured in the osteogenic induction medium containing GOQDs. (a) mRNA extracted at 7 days. (b) mRNA extracted at 14 days. (c) Protein extracted at 14 days. (d) Quantification of protein levels. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 vs. the control-si group. # p < 0.05, ## p < 0.01, ### p < 0.001, and #### p < 0.0001 vs. the β -catenin-si group.

Article Snippet: SHEDs were seeded into a culture flask, and when they reached 50% confluence, they were treated with DMEM containing enhanced green fluorescence protein-labeled (EGFP) β -catenin small interfering RNA (siRNA) lentivirus (Shanghai Genechem Co., Ltd., Shanghai, China). siRNA against β -catenin GTATTTGAAGTATACCATA and a nonspecific shRNA construct were designed and cloned onto an hU6-MCS-CMV-EGFP vector.

Techniques: Cell Culture